LOCUS       40924_31660       14060 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB304 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14060)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 14060)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14060)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    334..458
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        495..525
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             579..1235
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1580..1882
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1926..2050)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2085..3893
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3967..4219
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4253..4269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4277..4293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4301..4331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4346..4367)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(4452..4627)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(4635..6671)
                     /codon_start=1
                     /gene="mALS"
                     /product="acetolactate synthase"
                     /label=mALS
                     /note="from Marchantia polymorpha; contains triple
                     amino-acid subustitutions (P207S/R208S/W582L)"
                     /protein_id="BAS49887.1"
                     /translation="MASISGCAAVPLSIASLEKVQPSARSCCKGAKELKVRSGFFSGAA
                     LRGHAYNTSRKTERSLEVKAQAVSEKIQSAVDVSGPRLSSGKSLVAPTPSDFQSRYKDD
                     EPRKGADILVEALEREGIRTVFAYPGGASMEIHQALTRSSSIRNILCRHEQGEIFAAEG
                     YAKSSGRVGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVSSRMIGTDAFQETPIV
                     EVTRSITKHNYLVMNIDDIPRVIREAFFLAASGRPGPVLVDIPKDVQQQMAIPDWSQPM
                     QLKGYLQRLPRPPEPTLIEQIIRLISSSKKPVVYSGGGCNHASEELREFVELTGIPITS
                     TLMGLGAYPESDPLSLKMLGMHGTVYANYAVDQADLLLAFGVRFDDRVTGKLETFASRS
                     SIVHIDIDPAEIGKNKQPHVSMCADVKPALSEINALLKSQKRTFDFSNWVAELEEQKQK
                     WPMKFPVFEDAIAPQSAIQTLYELTGGNAIISTGVGQHQMWAAQWFKYNEPRRWLTSGG
                     LGAMGFGLPSALGAAATQPDIPVIDIDGDGSFVMNVQELATIFVEKLPVKIMILNNQHL
                     GMVVQLEDRFYKANRAHTYLGDPDHEEQIYPDFCTVADGFKVPNARVVKKDELRAAIQT
                     MLDSPGPYLLDVIVPHQEHVLPMIPGGASFKEIITEGDGRTIY"
     gene            complement(4635..6671)
                     /gene="mALS"
                     /label=mALS
     promoter        complement(6711..7387)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(7582..7606)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8127..8915
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9164..9752
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9938..10078)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10422..10616)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10685..11755)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12187..12813)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaat caaacaagtt tgtacaaaaa agctgaacga
      361 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
      421 acataatact gtaaaacaca acatatccag tcactatggc ggcccctcga ggggccgcat
      481 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
      541 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
      601 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
      661 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
      721 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
      781 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
      841 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
      901 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
      961 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     1021 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1081 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1141 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1201 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
     1261 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
     1321 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
     1381 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
     1441 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
     1501 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
     1561 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
     1621 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
     1681 ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc cgtgaacttt acccggtggt
     1741 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
     1801 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
     1861 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
     1921 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
     1981 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
     2041 acaaagtggt tcgatctaga ggatccccgg gtggtcagtc ccttatgtta cgtcctgtag
     2101 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
     2161 aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg
     2221 ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgcgggca
     2281 acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
     2341 tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg
     2401 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
     2461 aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg
     2521 gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
     2581 actatgccgg aatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
     2641 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
     2701 tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
     2761 ctggacaagg cactagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
     2821 aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc
     2881 cgcttcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca
     2941 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat
     3001 tcgataacgt gctgatggtg cacgaccacg cattaatgga ctggattggg gccaactcct
     3061 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
     3121 tcgtggtgat tgatgaaact gctgctgtcg gctttaacct ctctttaggc attggtttcg
     3181 aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc agtcaacggg gaaactcagc
     3241 aagcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg
     3301 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
     3361 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg
     3421 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
     3481 accgttatta cggatggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
     3541 aaaaagaact tctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
     3601 gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc
     3661 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
     3721 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
     3781 acaagaaagg gatcttcact cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa
     3841 aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa caatgaatca
     3901 acaactctcc tggcgcacca tcgtcggcta cagcctcggg aattgctacc gagctcgaat
     3961 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     4021 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     4081 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     4141 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     4201 tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca tggtcatagc
     4261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc tgaattaggc
     4381 gcgcctattt ctgaattcaa cacattgcgg acgtttttaa tgtactgaat taacgccgaa
     4441 ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat
     4501 tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat
     4561 gagcgaaacc ctataggaac cctaaattcc cttatctggg aactactcac acattattat
     4621 ggagaaactc gaagttagta tatagtcctt ccatcaccct ctgtgataat ctccttaaaa
     4681 cttgctcccc caggaatcat aggcagaacg tgctcctggt ggggaactat cacatccagt
     4741 aaataaggac cgggagaatc tagcatagtt tgaattgctg ctcttagttc atccttcttc
     4801 accacacgcg catttggcac cttgaaaccg tctgcaactg tacagaagtc ggggtagatc
     4861 tgctcctcgt gatctggatc acctaagtaa gtatgtgctc tgttggcctt gtagaatcta
     4921 tcctccaatt gtacgaccat tcctagatgt tggttgttta agatcatgat cttcacgggt
     4981 aacttctcca caaaaattgt agccaactcc tgtacgttca tcacaaagct accatcgcca
     5041 tcgatgtcga tcacaggaat atctggttga gttgcggcag cccccaaagc agaaggaagt
     5101 ccgaatccca tggctccaag acctccagat gtcaaccaac gccgcggttc gttgtacttg
     5161 aaccactggg ctgcccacat ttgatgttgg ccaacaccag tagatatgat ggcatttcca
     5221 ccagtgagtt cgtacagcgt ctgtatggca gattgaggag caatggcatc ctcgaatacg
     5281 ggaaacttca ttggccattt ctgcttttgt tcttctagtt ctgccaccca gttggagaag
     5341 tcgaaagtcc tcttttgact cttcaagagg gcattgattt cacttagcgc aggtttcaca
     5401 tcagcacaca tggacacatg gggctgtttg ttctttccaa tttcagcagg gtcaatatcg
     5461 atatgaacga tgctggacct gctagcaaaa gtctccagtt taccagtcac tcgatcgtcg
     5521 aaacgcactc caaatgccaa caacagatct gcttggtcca cggcgtagtt ggcgtaaaca
     5581 gttccatgca ttccaagcat tttcaaactt aatggatcag actcgggata cgctcccaaa
     5641 cccattaaag tcgatgtgat aggaattccg gtaagctcga caaattccct caactcttcg
     5701 ctggcgtgat tgcaacctcc tccagaatac actacaggct tcttagaact agaaatgagt
     5761 cttataatct gctcgattaa cgttggttcc ggtggccgag gcagtcgttg taagtagccc
     5821 ttcagttgca ttggctgaga ccaatctgga atagccattt gctgttgcac gtccttggga
     5881 atatctacca agactgggcc cggcctacct gaagctgcca aaaagaaagc ctccctgatt
     5941 acgcgaggga tgtcatcaat gttcataaca aggtaattgt gcttggtgat cgatctcgta
     6001 acctccacaa ttggagtttc ttgaaaagca tcggtaccaa tcatcctact ggatacttga
     6061 cctgtaatgg ccactagtgg cacactgtcc aagagggcat cagcgagccc ggtaacaaga
     6121 ttggtcgctc caggaccgga tgtggctata caaaccccta ctcgtccaga cgatttagca
     6181 taaccttcag cagcgaaaat ctccccctgc tcgtggcgac agagaatgtt gcggatgctg
     6241 ctggaacgtg taagagcctg gtgaatctcc atagacgctc cgccgggata agcgaacacc
     6301 gttctaatcc cttctcgctc aagagcctcc accaaaatgt cggctccttt gcggggctcg
     6361 tcatccttgt agcgagactg aaagtcggac ggggttggtg caacgagtga cttcccgctg
     6421 ctaagccgtg gtccactcac atcgactgca gactggatct tctcgctaac agcttgggct
     6481 ttcacttcca gtgaacgctc agtcttccga gaggtgttgt atgcatgtcc cctcagagcg
     6541 gctcccgaga agaaaccact cctcaccttt agctctttag ctcccttgca acaagacctt
     6601 gccgatggct gcaccttctc caggctcgct atgctcaaag gcacagctgc gcatccacta
     6661 attgacgcca tgctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc
     6721 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
     6781 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
     6841 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     6901 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaattatg gcatttgtag
     6961 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
     7021 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
     7081 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttcaca
     7141 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     7201 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
     7261 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
     7321 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
     7381 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
     7441 agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa tacgcaaacc gaattcagaa
     7501 ataaattcag cctaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     7561 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     7621 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     7681 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     7741 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     7801 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc
     7861 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     7921 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     7981 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     8041 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     8101 gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc
     8161 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     8221 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     8281 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     8341 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     8401 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     8461 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     8521 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     8581 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     8641 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     8701 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     8761 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     8821 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     8881 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca
     8941 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt
     9001 gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag
     9061 ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt
     9121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9301 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9781 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9841 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9901 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     9961 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
    10021 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
    10081 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
    10141 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
    10201 accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg
    10261 cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag
    10321 cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag
    10381 gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg
    10441 ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt
    10501 ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt
    10561 acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa
    10621 agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac
    10681 attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg
    10741 ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca
    10801 gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga
    10861 tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga
    10921 gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg
    10981 ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact
    11041 ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg
    11101 ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg
    11161 tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc
    11221 ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct
    11281 tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt
    11341 cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg
    11401 gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg
    11461 ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa
    11521 aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg
    11581 gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg
    11641 cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt
    11701 catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga
    11761 ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac
    11821 gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg
    11881 ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt
    11941 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc
    12001 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga
    12061 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg
    12121 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt
    12181 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc
    12241 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac
    12301 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac
    12361 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct
    12421 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat
    12481 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac
    12541 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc
    12601 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc
    12661 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc
    12721 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc
    12781 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt
    12841 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat
    12901 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc
    12961 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg
    13021 ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa
    13081 aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct
    13141 cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc
    13201 tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat
    13261 gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc
    13321 gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg
    13381 ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg
    13441 caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg
    13501 gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta
    13561 ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc
    13621 gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct
    13681 cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct
    13741 ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg
    13801 cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa
    13861 gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca
    13921 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
    13981 ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
    14041 atggacgaac ggataaacct
//
